Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Aug 22, 2025

Viability of Bioprinted Cellular Constructs Using a Three Dispenser Cartesian Printer

Viability of Bioprinted Cellular Constructs Using a Three Dispenser Cartesian Printer

Published on: September 22, 2015

Tunable and Compartmentalized Multimaterial Bioprinting for Complex Living Tissue Constructs.

Shabir Hassan^1,2, Eduardo Gomez-Reyes^1,3, Eduardo Enciso-Martinez^1,3

¹Division of Engineering in Medicine, Department of Medicine, Harvard Medical School, and Brigham and Women's Hospital, Cambridge, Massachusetts 02139, United States.

ACS Applied Materials & Interfaces

|November 8, 2022

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Zein-Ceria Hybrid Microparticles Enable Long-Term ROS-Scavenging Oxygenation for Osteogenic Microtissues Engineering.

Advanced materials (Deerfield Beach, Fla.)·2026

Same author

From Waste to Superhydrophobic Marine Armor: Bionic Shark Skin Coating Achieves Recyclable Oil-Water Separation with Exceptional Stability.

Langmuir : the ACS journal of surfaces and colloids·2026

Same author

Comparative study on pollution characteristics and ecological risk in Xiangshan Bay and its adjacent eastern sea area.

Marine pollution bulletin·2026

Same author

Rapid and non-destructive detection of hollow defects in pecans by near-infrared spectroscopy combined with multimodal data fusion and deep learning.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2026

Same author

Mass-Produced and High-Performance Nanowell Biosensor Fabricated via Semiconductor Manufacturing for Rapid and Accurate COVID-19 Diagnosis in the Clinical Field.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same author

Tailorable Biofunctionalization of Poly(acrylamide) Hydrogels via Firefly Luciferin-Bioinspired Click Ligation Accelerates Cell Attachment, Spreading, and Proliferation.

ACS applied materials & interfaces·2026

This study introduces a novel multimaterial bioprinting technique using colloidal gels to create complex, implantable tissue constructs. The method ensures structural integrity and promotes cell infiltration for enhanced in vivo performance.

Area of Science:

Biotechnology
Tissue Engineering
Materials Science

Background:

Developing implantable tissue constructs with complex microarchitectures and inherent heterogeneity remains a significant challenge.
Maintaining structural integrity of 3D printed constructs in vivo is crucial for successful implantation.

Purpose of the Study:

To present a combinational multimaterial and embedded bioprinting approach for fabricating complex, implantable tissue constructs.
To demonstrate the ability of these constructs to retain their 3D shape and promote cell infiltration in vivo.

Main Methods:

Utilized a microfluidics-based single nozzle printhead with computer-controlled pneumatic pressure valves for precise bioink voxelation and rapid switching.
Incorporated self-healing and biodegradable colloidal gels as support baths to enhance spatial organization, printing fidelity, and speed.

Keywords:

3D bioprinting colloidal hydrogels compartmentalized bioprinting multimaterial extrusion bioprinting vascular scaffolds

More Related Videos

Bioprinting Cellularized Constructs Using a Tissue-specific Hydrogel Bioink

Bioprinting Cellularized Constructs Using a Tissue-specific Hydrogel Bioink

Published on: April 21, 2016

Author Spotlight: Understanding Chronic Lung Diseases Using 3D Printed Phototunable Hydrogels

Author Spotlight: Understanding Chronic Lung Diseases Using 3D Printed Phototunable Hydrogels

Published on: June 30, 2023

Related Experiment Videos

Last Updated: Aug 22, 2025

Viability of Bioprinted Cellular Constructs Using a Three Dispenser Cartesian Printer

Viability of Bioprinted Cellular Constructs Using a Three Dispenser Cartesian Printer

Published on: September 22, 2015

Bioprinting Cellularized Constructs Using a Tissue-specific Hydrogel Bioink

Bioprinting Cellularized Constructs Using a Tissue-specific Hydrogel Bioink

Published on: April 21, 2016

Author Spotlight: Understanding Chronic Lung Diseases Using 3D Printed Phototunable Hydrogels

Author Spotlight: Understanding Chronic Lung Diseases Using 3D Printed Phototunable Hydrogels

Published on: June 30, 2023

Developed multicompartment microfibers and acellular complex geometries using up to seven bioinks within the colloidal gel support.

Main Results:

Successfully printed complex geometries including multicompartment microfibers (solid, core-shell, donut) and acellular structures (pyramids, spirals, vessels).
Fabricated vascularized liver constructs exhibiting albumin secretion and skeletal muscle constructs with bundled muscle mimic fibers.
Demonstrated that the colloidal gel's interconnected microporous networks maintain construct geometry and facilitate rapid host cell invasion in vitro and in vivo.

Conclusions:

The presented bioprinting approach enables the fabrication of complex, implantable tissue constructs with high fidelity and structural integrity.
The use of colloidal gels as support baths is critical for achieving spatial organization, improving printing performance, and providing a native-like microenvironment for cells.
The developed technique holds significant promise for advancing the field of regenerative medicine and creating functional tissue replacements.