Synthesis of Nickel-Chitosan Nanoparticles for Controlling Blast Diseases in Asian Rice

Rice blast caused by Pyricularia oryzae is one of most devastating fungal diseases in rice, reducing the annual yield of rice worldwide. As an alternative to fungicide for curbing rice blast, synthesis of nickel-chitosan nanoparticles (Ni-Ch NPs) was performed with nickel chloride and assessed its efficacy in inflating plant growth and hindrance of Pyricularia oryzae (blast pathogen). Characterization of Ni-Ch NPs from SEM, TEM, and DLS analyses showed smooth- and spherical-shaped nanoparticles in the range of 20-70 nm. Colloidal stability of NPs was revealed from Zeta potential exhibiting polydispersity index of 0.22. EDX spectroscopy corroborated the presence of nickel (14.05%) in synthesized Ni-Ch NPs. A significant increase in germination and growth attributes in terms of shoot and root length and number of lateral roots over control was observed in paddy seeds on the treatment with Ni-Ch NPs. Furthermore, the application of NPs in paddy plants under glasshouse condition demonstrated a remarkable improvement in plant growth. Protein profiling of NP-treated plants revealed new polypeptides (Rubisco units) enlightening the enhanced photosynthetic rate. Also, Asian rice exhibited reduced blast symptoms on leaves treated with NPs under glasshouse condition while displaying 64% mycelia inhibition in Petri plates. All these results suggest that nickel-chitosan nanoparticles could be exploited as an effective plant growth promoter cohort in controlling rice blast disease.