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Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

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In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or...
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Eu-Chelate Polystyrene Microsphere-Based Lateral Flow Immunoassay Platform for hs-CRP Detection.

Birui Jin1,2, Zhiguo Du1, Chuyao Zhang1

  • 1School of Materials and Chemical Engineering, Xi'an Technological University, Xi'an 710021, China.

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|November 10, 2022
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Summary

A new high-sensitivity C-reactive protein (hs-CRP) test using europium-chelate polystyrene microspheres (EuPSM) offers rapid, user-friendly detection. This assay is crucial for early identification of inflammation and chronic diseases.

Keywords:
fluorescence analysishs-CRP detectionpaper microfluidicssensitivity enhancement

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Immunodiagnostics

Background:

  • Inflammation from infections poses a global health risk.
  • Blood C-reactive protein (CRP) indicates inflammation, with subtle changes potentially signaling chronic diseases.
  • A need exists for sensitive, rapid, and affordable high-sensitivity CRP (hs-CRP) detection.

Purpose of the Study:

  • To develop a novel europium-chelate polystyrene microsphere (EuPSM)-based lateral flow immunoassay (LFIA) for quantitative hs-CRP detection.
  • To optimize the EuPSM-based LFIA for enhanced sensitivity and a wide detection range.
  • To validate the clinical applicability of the developed LFIA platform.

Main Methods:

  • Development of a EuPSM-based lateral flow immunoassay (LFIA).
  • Integration of the LFIA with a benchtop fluorescence analyzer.
  • Optimization of antibody density on EuPSM and validation with clinical serum samples.

Main Results:

  • Achieved a limit of detection of 0.76 pg/mL for hs-CRP.
  • Established a broad detection range from 0.025 ng/mL to 250 ng/mL.
  • Demonstrated high consistency with standard clinical methods in serum sample analysis.

Conclusions:

  • The EuPSM-based LFIA is a highly sensitive and rapid platform for hs-CRP detection.
  • The assay shows significant potential for early disease detection and monitoring.
  • The platform is adaptable for the sensitive detection of other biomarkers.