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The Dimethylmethylene Blue Assay (DMMB) for the Quantification of Sulfated Glycosaminoglycans
Yann D Ladner1,2, Mauro Alini3, Angela R Armiento4
1AO Research Institute Davos, Davos Platz, Switzerland.
Methods in Molecular Biology (Clifton, N.J.)
|November 10, 2022
Abstract:
The 1,9-dimethylmethylene blue (DMMB) assay enables the detection of sulfated glycosaminoglycans (sGAGs). This assay can be used to quickly quantify the sGAG content in a large number of samples using spectrophotometry. While this widespread assay appears straightforward, there are certain pitfalls that need to be considered.
