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Updated: Aug 22, 2025

Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors
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Sulfone Rhodamines for Voltage Imaging.

Benjamin K Raliski1, Dong Min Mun1, Evan W Miller1,2,3

  • 1Department of Chemistry, University of California, Berkeley, 94720-1460, Berkeley, CA, USA.

Chemistry, an Asian Journal
|November 10, 2022
PubMed
Summary
This summary is machine-generated.

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New voltage-sensitive fluorophores, Sulfone Rhodamine Voltage Reporters (SuRhoVRs), absorb and emit near-infrared light for improved neuronal activity monitoring. These photostable indicators track membrane potential changes in neurons.

Area of Science:

  • Neuroscience
  • Biophysics
  • Chemical Biology

Background:

  • Fluorescent indicators are crucial for monitoring neuronal activity by detecting changes in biological membrane potentials.
  • Near-infrared (NIR) fluorescent indicators offer advantages due to deeper tissue penetration and reduced excitation of biological molecules.
  • Existing NIR voltage-sensitive dyes have limitations in sensitivity, photostability, or spectral range.

Purpose of the Study:

  • To develop novel voltage-sensitive fluorophores that absorb and emit in the near-infrared window (>700 nm).
  • To create voltage-sensitive dyes based on sulfone rhodamine chromophores integrated into a voltage-sensitive scaffold.
  • To evaluate the performance of these novel dyes in sensing membrane potential changes in neuronal cells.

Main Methods:

Keywords:
fluorescencefluorescent probesmembrane potentialresponsive indicators

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  • Incorporation of sulfone rhodamine chromophores into a voltage-sensitive scaffold.
  • Synthesis and characterization of Sulfone Rhodamine Voltage Reporters (SuRhoVRs).
  • Assessment of SuRhoVRs' membrane partitioning, voltage sensitivity, and photostability in dissociated rat hippocampal neurons.

Main Results:

  • Developed novel voltage-sensitive fluorophores (SuRhoVRs) absorbing and emitting above 700 nm.
  • SuRhoVRs effectively partition into cell membranes and exhibit sensitivity to membrane potential changes.
  • The most sensitive SuRhoVR derivative demonstrated excellent photostability and successfully tracked neuronal membrane potential dynamics.

Conclusions:

  • Sulfone Rhodamine Voltage Reporters (SuRhoVRs) represent a promising new class of NIR voltage-sensitive dyes.
  • SuRhoVRs offer enhanced optical properties for monitoring neuronal activity with improved penetration and reduced phototoxicity.
  • These novel fluorophores have potential applications in advanced neuroimaging and electrophysiology studies.