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Urinary Bladder Patch Made with Decellularized Vein Scaffold Seeded with Adipose-Derived Mesenchymal Stem Cells:
Tadeu Ravazi Piovesana1, Lenize da Silva Rodrigues1,2, Ana Livia de Carvalho Bovolato2
1Department of Surgery and Orthopedics, Botucatu Medical School, São Paulo State University-UNESP, Botucatu 18618-687, Brazil.
Biomedicines
|November 11, 2022
Summary
Tissue regeneration in rabbit bladders improved with decellularized vein scaffolds seeded with adipose-derived mesenchymal stem cells (ASCs). ASCs accelerated urothelial epithelialization and tissue maturation, enhancing bladder repair.
Area of Science:
- Regenerative Medicine
- Biomaterials Science
- Urology
Background:
- Investigating tissue regeneration of the urinary bladder.
- Utilizing decellularized veins as scaffolds.
- Employing autologous adipose-derived mesenchymal stem cells (ASCs).
Purpose of the Study:
- To evaluate tissue regeneration of the urinary bladder.
- To assess the efficacy of decellularized vein scaffolds combined with ASCs.
- To analyze the impact of ASCs on luminal surfaces of the scaffold.
Main Methods:
- New Zealand rabbits (n=10) were divided into two groups: scaffold alone (G1) and scaffold with ASCs (G2).
- Decellularized vena cava scaffolds (1 cm²) were implanted.
- Specimens were analyzed after 21 days using histology (HE) and scanning electron microscopy (SEM).
Main Results:
- Urinary bladder integrity was maintained in both groups.
- Group G2 (scaffold plus ASCs) showed a superior regenerative process compared to G1.
- Enhanced urothelial epithelialization and mature mucosa/submucosa fibroblasts were observed in G2, with notable urothelial villi via SEM.
Conclusions:
- Decellularized vena cava scaffolds effectively maintained urinary bladder integrity.
- ASCs significantly accelerated the regenerative process.
- The study highlights improved urothelial epithelization and fibroblast maturity with ASC-seeded scaffolds.
