Human Brain Organoids-on-Chip: Advances, Challenges, and Perspectives for Preclinical Applications.

Area of Science:

• Neuroscience
• Biotechnology
• Pharmacology

Background:

• Urgent need for predictive in vitro models in neuroscience for disease modeling and drug discovery.
• Cerebral organoids recapitulate human brain features, serving as powerful tools for biological process deciphering.
• Microfluidic technologies combined with cerebral organoids create brain organoids-on-chips for 3D neuronal microenvironment modeling.

Purpose of the Study:

• To review the advantages of cerebral organoids for disease modeling.
• To explore how microfluidic integration enhances in vitro model reproducibility and quality.
• To highlight the potential of brain organoids-on-chips for pharmacological applications.

Main Methods:

• Review of current literature on cerebral organoids and microfluidic systems.
• Focus on the combination of cerebral organoids with microfluidic technologies (brain organoids-on-chips).
• Discussion of requirements for industrial adoption: reproducibility, quality, automation, and high-throughput screening compatibility.

Main Results:

• Cerebral organoids provide a robust platform for studying complex neurological processes.
• Brain organoids-on-chips improve the structural and functional maturation of in vitro brain models.
• Microfluidic integration enhances the reproducibility and quality of cerebral organoids.

Conclusions:

• Brain organoids-on-chips represent a significant advancement for preclinical neuroscience research.
• This technology offers a promising in vitro approach for drug discovery and target validation.
• Further development is needed to meet industry demands for automation and high-throughput screening.