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Updated: Aug 22, 2025

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects
Published on: February 8, 2014
Classification of Holograms with 3D-CNN
Dániel Terbe1, László Orzó1, Ákos Zarándy1
1Institute for Computer Science and Control, H-1111 Budapest, Hungary.
Abstract:
A hologram, measured by using appropriate coherent illumination, records all substantial volumetric information of the measured sample. It is encoded in its interference patterns and, from these, the image of the sample objects can be reconstructed in different depths by using standard techniques of digital holography. We claim that a 2D convolutional network (CNN) cannot be efficient in decoding this volumetric information spread across the whole image as it inherently operates on local spatial features. Therefore, we propose a method, where we extract the volumetric information of the hologram by mapping it to a volume-using a standard wavefield propagation algorithm-and then feed it to a 3D-CNN-based architecture. We apply this method to a challenging real-life classification problem and compare its performance with an equivalent 2D-CNN counterpart. Furthermore, we inspect the robustness of the methods to slightly defocused inputs and find that the 3D method is inherently more robust in such cases. Additionally, we introduce a hologram-specific augmentation technique, called hologram defocus augmentation, that improves the performance of both methods for slightly defocused inputs. The proposed 3D-model outperforms the standard 2D method in classification accuracy both for in-focus and defocused input samples. Our results confirm and support our fundamental hypothesis that a 2D-CNN-based architecture is limited in the extraction of volumetric information globally encoded in the reconstructed hologram image.
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