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Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
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Related Experiment Video

Updated: Aug 22, 2025

Infinium Assay for Large-scale SNP Genotyping Applications

Infinium Assay for Large-scale SNP Genotyping Applications

Published on: November 19, 2013

CAPG: comprehensive allopolyploid genotyper.

Roshan Kulkarni¹, Yudi Zhang², Steven B Cannon³

¹Department of Agronomy, Iowa State University, Ames, IA 50011, USA.

Bioinformatics (Oxford, England)

|November 11, 2022

Summary

This summary is machine-generated.

Genotyping by sequencing in allopolyploids is challenging due to homoeologous similarity. The Comprehensive Allopolyploid Genotyper (CAPG) tool accurately assigns reads to subgenomes, improving SNP calling in polyploid plants.

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FISH for Pre-implantation Genetic Diagnosis

FISH for Pre-implantation Genetic Diagnosis

Published on: February 23, 2011

Area of Science:

Genomics
Plant genetics
Bioinformatics

Background:

Genotyping by sequencing (GBS) is crucial for plant genetic variation studies.
Allopolyploid plants present challenges in GBS due to homoeologous similarity, complicating read assignment and SNP detection.
Existing methods struggle with accurate read assignment, leading to false heterozygous calls.

Purpose of the Study:

To develop a novel method for accurate genotyping in allopolyploid plants.
To improve the resolution of subgenomic origin for sequencing reads in polyploids.
To enhance the accuracy of single nucleotide polymorphism (SNP) calling in allopolyploid genomes.

Main Methods:

Introduced the Comprehensive Allopolyploid Genotyper (CAPG) tool.
Formulated an explicit likelihood to weight read alignments against subgenomic references.
Applied CAPG to whole-genome resequencing data from allopolyploids.

Main Results:

CAPG effectively resolves read assignment to subgenomic origins.
Demonstrated superior performance of CAPG compared to standard methods like Genome Analysis Toolkit's HaplotypeCaller in allotetraploids.
Reduced false heterozygous calls by accurately distinguishing homoeologous SNPs.

Conclusions:

CAPG provides a robust solution for accurate genotyping in allopolyploid plants.
The method significantly improves the reliability of genetic variation analysis in polyploid species.
CAPG facilitates deeper insights into the genetic architecture of economically important polyploid crops.