Tragia plukenetii-Assisted Omega-Decenol as Potential Anticancer Agent: its Isolation, Characterization, and...

Area of Science:

• Phytochemistry
• Pharmacology
• Cancer Research

Background:

• Lung cancer is a leading cause of cancer-related mortality.
• There is a growing need for natural phytomedicines as alternatives to synthetic drugs.
• Tragia plukenetii, a plant from India's Euphorbiaceae family, is recognized for its medicinal properties.

Purpose of the Study:

• To isolate bioactive compounds from Tragia plukenetii leaves.
• To evaluate the anticancer properties of isolated compounds against lung cancer.

Main Methods:

• Compound isolation using Thin-Layer Chromatography (TLC).
• Structural elucidation via UV-Visible Spectrophotometry, FT-IR, 13C-NMR, and 1H-NMR.
• In vitro antioxidant (DPPH assay) and anticancer (MTT assay, FACS analysis) evaluations.
• Gas Chromatography-Mass Spectrometry (GC-MS) for active fraction analysis.
• In silico molecular docking against EGFR.

Main Results:

• Omega-decenol (OD) was isolated and identified from T. plukenetii.
• OD exhibited significant cytotoxic effects against the A549 lung cancer cell line (IC50 = 24 µg/ml).
• OD induced S-phase cell cycle arrest in A549 cells.
• In silico studies showed good binding affinity of OD against EGFR.

Conclusions:

• Omega-decenol isolated from T. plukenetii possesses potent anticancer activity against lung adenocarcinoma.
• OD demonstrates potential as a novel phytomedicine for lung cancer therapy.
• Further research into the molecular interactions of OD is warranted.