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Updated: Aug 22, 2025
High-Throughput Automated Multiplex Immunofluorescence Assays for Translational Research
Published on: June 10, 2025
Optimal CCN4 Immunofluorescence for Tissue Microarray
Pierre-Olivier Gaudreau1, Isabelle Cousineau2, John Stagg2,3
1Department of Oncology, Queen's University and Canadian Cancer Trials Group, Kingston, ON, Canada. p-ogaudreau@ctg.queensu.ca.
Abstract:
CCN4 (also known as WNT1-Inducible Signaling Pathway Protein 1 or WISP1) is a 367 amino acid, 40 kDa protein located on chromosome 8q24.1-8q24.3. Prior studies have provided support for a pro-inflammatory role for CCN4. We have shown recently that CCN4 expression is associated with advanced disease, epithelial-mesenchymal transition, and an inflamed tumor microenvironment in multiple solid tumors. We detail here the CCN4 tissue microarray immunofluorescence protocol related to these findings.
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