Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

10.9K
A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
10.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Neoadjuvant stereotactic body radiation therapy with durvalumab and oleclumab in ER<sup>+</sup>HER2<sup>-</sup> breast cancer: a randomized phase 2 trial.

Nature medicine·2026
Same author

CRI-CCTG-0003/IND.240 an immunotherapy platform study in platinum-resistant high grade serous ovarian cancer (IPROC): Sub-studies A and B: Durvalumab (D) + mecbotamab vedotin (BA3011) or ozuriftamab vedotin (BA3021).

Cancer treatment and research communications·2026
Same author

Descriptive study of stromal expression of CD73 in ductal carcinoma in situ of the breast.

Discover oncology·2026
Same author

Targeting PAR-2 with a negative allosteric modulator increases tumor antigen presentation and potentiates anti-PD-1 immunotherapy.

Journal for immunotherapy of cancer·2026
Same author

The deubiquitinase USP17 regulates the expression and activity of the oncogenic driver β-catenin in colorectal cancer.

Oncogene·2026
Same author

Effects of serotonergic drugs on immune checkpoint inhibitor response: a pooled analysis of individual patient data from four Canadian Cancer Trials Group (CCTG) trials.

Immunotherapy·2025
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Aug 22, 2025

High-Throughput Automated Multiplex Immunofluorescence Assays for Translational Research
09:12

High-Throughput Automated Multiplex Immunofluorescence Assays for Translational Research

Published on: June 10, 2025

362

Optimal CCN4 Immunofluorescence for Tissue Microarray.

Pierre-Olivier Gaudreau1, Isabelle Cousineau2, John Stagg2,3

  • 1Department of Oncology, Queen's University and Canadian Cancer Trials Group, Kingston, ON, Canada. p-ogaudreau@ctg.queensu.ca.

Methods in Molecular Biology (Clifton, N.J.)
|November 12, 2022
PubMed
Summary
This summary is machine-generated.

CCN4 protein is linked to advanced cancer, cell changes, and inflammation. This study details the protocol used to examine CCN4's role in solid tumors.

Keywords:
CCN proteinsCCN4IFImmunofluorescenceTMATissue microarrayWISP1

More Related Videos

Author Spotlight: Multiplex Immunofluorescence Combined with Spatial Image Analysis for the Clinical and Biological Assessment of the Tumor Microenvironment
06:05

Author Spotlight: Multiplex Immunofluorescence Combined with Spatial Image Analysis for the Clinical and Biological Assessment of the Tumor Microenvironment

Published on: June 2, 2023

8.0K
Author Spotlight: Efficient Detection of Immune Cell-Infiltration in Cancer Tissues Using Fluorescent Immunohistochemistry
04:21

Author Spotlight: Efficient Detection of Immune Cell-Infiltration in Cancer Tissues Using Fluorescent Immunohistochemistry

Published on: January 26, 2024

855

Related Experiment Videos

Last Updated: Aug 22, 2025

High-Throughput Automated Multiplex Immunofluorescence Assays for Translational Research
09:12

High-Throughput Automated Multiplex Immunofluorescence Assays for Translational Research

Published on: June 10, 2025

362
Author Spotlight: Multiplex Immunofluorescence Combined with Spatial Image Analysis for the Clinical and Biological Assessment of the Tumor Microenvironment
06:05

Author Spotlight: Multiplex Immunofluorescence Combined with Spatial Image Analysis for the Clinical and Biological Assessment of the Tumor Microenvironment

Published on: June 2, 2023

8.0K
Author Spotlight: Efficient Detection of Immune Cell-Infiltration in Cancer Tissues Using Fluorescent Immunohistochemistry
04:21

Author Spotlight: Efficient Detection of Immune Cell-Infiltration in Cancer Tissues Using Fluorescent Immunohistochemistry

Published on: January 26, 2024

855

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • CCN4, also known as WNT1-Inducible Signaling Pathway Protein 1 (WISP1), is a protein implicated in cellular processes.
  • Previous research suggests CCN4 has a pro-inflammatory role.
  • Our recent findings associate CCN4 expression with advanced disease, epithelial-mesenchymal transition, and an inflamed tumor microenvironment in solid tumors.

Purpose of the Study:

  • To detail the CCN4 tissue microarray immunofluorescence protocol.
  • To provide a reproducible method for analyzing CCN4 expression in solid tumors.

Main Methods:

  • Tissue microarray construction.
  • Immunofluorescence staining for CCN4 detection.
  • Microscopic analysis of stained tissue sections.

Main Results:

  • Established a reliable protocol for CCN4 detection using immunofluorescence on tissue microarrays.
  • Demonstrated the feasibility of analyzing CCN4 expression patterns in relation to tumor characteristics.

Conclusions:

  • The detailed protocol facilitates further investigation into CCN4's role in cancer.
  • This method supports research linking CCN4 to advanced disease and tumor microenvironment inflammation.