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Evaluation of the Molecular Interaction Between CCN Protein and Its Binding Partners: A Solid-Phase Binding Assay and
Eriko Aoyama1, Masaharu Takigawa2
1Advanced Research Center for Oral and Craniofacial Sciences, Okayama University Dental School, Okayama, Japan. eaoyama@cc.okayama-u.ac.jp.
Abstract:
CCN proteins are known to bind to various growth factors, cytokines, and membrane proteins. Since these bindings are closely involved in the function of CCN proteins, the analysis of the binding partners is the first step toward understanding the mechanisms of actions of CCN proteins. This chapter describes two approaches used for such analyses: a solid-phase binding assay, which is suitable for confirming the binding easily because of its simplicity and cost advantage, and a surface plasmon resonance assay, which can determine the binding affinities between CCN proteins and their partners.
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