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Interlaboratory comparisons using ultrahigh resolution mass spectrometry (UHR-MS) with direct infusion (DI) are crucial for quality control. A global study found that five laboratories demonstrated comparable performance, establishing a reference for future blood plasma analysis ring trials.

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Area of Science:

  • Analytical Chemistry
  • Metabolomics
  • Mass Spectrometry

Background:

  • Ultrahigh resolution mass spectrometry (UHR-MS) coupled with direct infusion (DI) electrospray ionization enables rapid, untargeted profiling of complex chemical systems.
  • Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometers are valuable for molecular formula assignment in complex samples, but interlaboratory comparability is not well-established.
  • Standardized methods and interlaboratory comparisons are essential for applying FT-ICR MS in quality control, environmental studies, and clinical diagnostics.

Purpose of the Study:

  • To assess the comparability of data generated by diverse FT-ICR mass spectrometers with varying technical specifications.
  • To evaluate the reproducibility of direct infusion (DI) FT-ICR MS analyses across multiple laboratories.
  • To establish a framework for a future DI-FT-ICR MS ring trial for blood plasma analysis.

Main Methods:

  • A preliminary study involving 17 global laboratories analyzing identical human blood plasma and Standard Reference Material (SRM1950) samples.
  • Samples were analyzed using direct infusion (DI) electrospray ionization coupled with FT-ICR mass spectrometry.
  • Samples included varying dilutions and pesticide spikes to challenge instrument performance and comparability.

Main Results:

  • A subset of five laboratories, despite diverse instrumental characteristics, exhibited comparable and representative performance across all experimental conditions.
  • The study identified a cluster of instruments capable of generating reproducible results for blood plasma analysis.
  • Variability in instrument tuning and technical specifications impacts data comparability, but a core group demonstrated consistent performance.

Conclusions:

  • A core group of five laboratories demonstrated sufficient comparability for FT-ICR MS data to serve as a reference point.
  • This finding provides a foundation for designing a robust ring trial for direct infusion FT-ICR MS analysis of blood plasma.
  • Establishing interlaboratory comparability is a critical step towards the routine application of UHR-MS in quality control and diagnostics.