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In Vitro Comparison Between Ovine and Human Recombinant Hyaluronidase on Hyaluronic Acid Fillers
Britton P Beatrous1, Peter H Liddell2, Lindsay M Turner3
1Department of Otolaryngology-Head and Neck Surgery, University of Mississippi Medical Center, Jackson, Mississippi, USA.
Facial Plastic Surgery & Aesthetic Medicine
|November 14, 2022
Summary
Human recombinant hyaluronidase (HAse) more effectively degrades hyaluronic acid (HA) fillers than ovine HAse. This study compared the in vitro degradation rates of various HA fillers by both HAse forms, finding the human form superior.
Area of Science:
- Dermatology
- Biochemistry
- Cosmetic Science
Background:
- Injectable hyaluronic acid (HA) fillers are widely used for facial aesthetics and anti-aging treatments.
- Hyaluronidase (HAse) enzymes, derived from ovine or human sources, are employed to manage or reverse HA filler effects.
- Current clinical practice often uses ovine and human recombinant HAse interchangeably, with limited data on their comparative efficacy.
Purpose of the Study:
- To quantitatively compare the degradation efficacy of ovine versus human recombinant hyaluronidase (HAse) on diverse hyaluronic acid (HA) filler formulations.
- To investigate potential differences in the enzymatic activity of HAse from different species against various HA filler substrates in an in vitro setting.
Main Methods:
- A colorimetric assay was utilized to measure the degradation of fixed quantities of nine distinct HA filler products.
- Increasing concentrations of either ovine or human recombinant HAse were applied to the HA filler samples.
- Absorbance levels of the degraded products were analyzed to determine and compare degradation rates.
Main Results:
- Human recombinant HAse demonstrated a statistically significant greater overall degradation of hyaluronic acid fillers compared to ovine HAse (p=0.014).
- The study observed a consistent trend where the human HAse formulation exhibited higher efficacy across the tested HA filler types.
Conclusions:
- Human recombinant hyaluronidase is more effective on average than ovine hyaluronidase in degrading hyaluronic acid fillers in vitro.
- These findings suggest that the source of recombinant hyaluronidase may influence its efficacy in clinical applications involving HA filler reversal.
