Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Peptide Bonds02:43

Peptide Bonds

75.4K
A peptide bond covalently attaches amino acids through a dehydration reaction. One amino acid's carboxyl group and another amino acid's amino group combine, releasing a water molecule. The resulting bond is the peptide bond. The products that such linkages form are peptides. As more amino acids join this growing chain, the resulting chain is a polypeptide. Each polypeptide has a free amino group at one end. This end has the N-terminal, or the amino-terminal, and the other end has a free...
75.4K
Protein Folding01:25

Protein Folding

8.3K
Proteins are chains of amino acids linked together by peptide bonds. Upon synthesis, a protein folds into a three-dimensional conformation, critical to its biological function. Interactions between its constituent amino acids guide protein folding, and hence the protein structure is primarily dependent on its amino acid sequence.
Protein Structure Is Critical to Its Biological Function
Proteins perform a wide range of biological functions such as catalyzing chemical reactions, providing...
8.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<i>De novo</i> grafted coiled-coil peptides as p53/<i>h</i>DM2 inhibitors.

RSC chemical biology·2026
Same author

The open-source Masala software suite: Facilitating rapid methods development for synthetic heteropolymer design.

Methods in enzymology·2025
Same author

Veraguamide E, a Marine Cyanobacterial Depsipeptide Targeting σ<sub>2</sub>R/TMEM97: Chemical and Neurobiological Characterization.

Journal of natural products·2025
Same author

Development of Insulin-Like Growth Factor Mimetic Materials.

Advanced biology·2025
Same author

The open-source Masala software suite: Facilitating rapid methods development for synthetic heteropolymer design.

bioRxiv : the preprint server for biology·2025
Same author

Neurobiological and Chemical Characterization of the Cyanobacterial Metabolite Veraguamide E.

bioRxiv : the preprint server for biology·2025
Same journal

Isolation of Mesenchymal Stem Cell-Derived Extracellular Vesicles.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Modeling Melanoma Immune Surveillance by CAR-T Cells in Human Skin Organoids.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Stepwise Optimization of a Matrigel-Based In Vitro Angiogenesis Assay for Reproducible and Quantifiable 2D-Tube Formation Using HUVECs.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Quantifying Mechanical Properties of Fresh Ovarian Tissue with Optical Brillouin Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

3D Chromatin Architecture During Early Development: New Methods and New Findings.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Metabolic Plasticity in Embryogenesis Throughout the Lens of NAD<sup></sup>.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Aug 22, 2025

Constructing Thioether/Vinyl Sulfide-tethered Helical Peptides Via Photo-induced Thiol-ene/yne Hydrothiolation
11:09

Constructing Thioether/Vinyl Sulfide-tethered Helical Peptides Via Photo-induced Thiol-ene/yne Hydrothiolation

Published on: August 1, 2018

10.8K

How to Design Peptides.

Joseph Dodd-O1, Amanda M Acevedo-Jake1, Abdul-Rahman Azizogli2

  • 1Department of Biomedical Engineering, New Jersey Institute of Technology, Newark, NJ, USA.

Methods in Molecular Biology (Clifton, N.J.)
|November 14, 2022
PubMed
Summary
This summary is machine-generated.

This study introduces a generalized computational method for designing novel peptides to target specific proteins. The approach, utilizing the Rosetta software suite, empowers beginners to create peptide ligands for drug discovery and biological studies.

Keywords:
Bioactive peptidePeptide designPeptide inhibitorsPeptide ligandPeptide–protein interactionRosetta scripts

More Related Videos

Constructing Cyclic Peptides Using an On-Tether Sulfonium Center
07:11

Constructing Cyclic Peptides Using an On-Tether Sulfonium Center

Published on: September 28, 2022

2.8K
Formation of Ordered Biomolecular Structures by the Self-assembly of Short Peptides
07:26

Formation of Ordered Biomolecular Structures by the Self-assembly of Short Peptides

Published on: November 21, 2013

13.0K

Related Experiment Videos

Last Updated: Aug 22, 2025

Constructing Thioether/Vinyl Sulfide-tethered Helical Peptides Via Photo-induced Thiol-ene/yne Hydrothiolation
11:09

Constructing Thioether/Vinyl Sulfide-tethered Helical Peptides Via Photo-induced Thiol-ene/yne Hydrothiolation

Published on: August 1, 2018

10.8K
Constructing Cyclic Peptides Using an On-Tether Sulfonium Center
07:11

Constructing Cyclic Peptides Using an On-Tether Sulfonium Center

Published on: September 28, 2022

2.8K
Formation of Ordered Biomolecular Structures by the Self-assembly of Short Peptides
07:26

Formation of Ordered Biomolecular Structures by the Self-assembly of Short Peptides

Published on: November 21, 2013

13.0K

Area of Science:

  • Biochemistry
  • Computational Biology
  • Drug Discovery

Background:

  • Protein engineering advances enable novel therapeutic and augmentation strategies.
  • Peptides offer advantages over larger proteins for targeted signaling when combined with carriers.
  • Computational design is crucial for developing new peptide-based therapeutics.

Purpose of the Study:

  • To present a generalized computational method for designing novel peptides.
  • To provide a step-by-step guide for beginners using the Rosetta software suite.
  • To facilitate the creation of peptide ligands for targeting specific proteins.

Main Methods:

  • Development of a script protocol for iterative peptide sequence optimization and screening.
  • Utilizing file repositories, databases, and the Rosetta software suite.
  • Employing RosettaScripts, an XML interface, for sequential and repeatable function execution.

Main Results:

  • A generalized method for designing novel peptides is presented.
  • Beginners can design their first peptide ligand using the provided protocol.
  • The method enables peptides to be designed for interaction with various enzymes and receptors.

Conclusions:

  • The presented computational design strategy can accelerate peptide drug discovery.
  • This approach may foster collaborations between computational design and experimental screening methods like AI/ML and phage display.
  • The method is applicable to diverse biological targets, including chemokine-receptor interactions.