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Generation of Bone Marrow Chimeras

Yayi Gao1, Rémy Bosselut2

  • 1Laboratory of Immune Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.

Methods in Molecular Biology (Clifton, N.J.)
|November 14, 2022
PubMed

Bone marrow chimeras are widely used in immunological studies, to dissect the contributions of hematopoietic and non-hematopoietic cells in immune cell development or functions, to quantify the impact of a given mutation, or in preclinical studies for hematopoietic stem cell transplantation. Here we describe a set of procedures for the generation of bone marrow chimeras.

Keywords:
Bone marrow chimerasHematopoietic cellsImmune reconstitutionT cell development

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