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Updated: Aug 22, 2025

Long-term Silencing of Intersectin-1s in Mouse Lungs by Repeated Delivery of a Specific siRNA via Cationic Liposomes. Evaluation of Knockdown Effects by Electron Microscopy
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Natural Antisense Long Noncoding RNA HHIP-AS1 Suppresses Non-Small-Cell Lung Cancer Progression by Increasing HHIP

Yan Yang1, Yue Cheng2, Yanfei Mou2

  • 1Chongqing Key Laboratory of Translational Research for Cancer Metastasis and Individualized Treatment, Department of Medical Oncology, Chongqing University Cancer Hospital, Chongqing 400030, China.

Critical Reviews in Eukaryotic Gene Expression
|November 14, 2022
PubMed

Insights

Hedgehog-interacting protein antisense RNA 1 (HHIP-AS1) is downregulated in non-small-cell lung cancer (NSCLC). Overexpression of HHIP-AS1 inhibits NSCLC cell proliferation, migration, and invasion by stabilizing HHIP protein levels.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Non-small-cell lung cancer (NSCLC) presents high incidence and mortality rates.
  • Long noncoding RNAs (lncRNAs) are implicated in NSCLC development.
  • The role of Hedgehog-interacting protein antisense RNA 1 (HHIP-AS1) in NSCLC remains largely uncharacterized.

Purpose of the Study:

  • To investigate the function of HHIP-AS1 in non-small-cell lung cancer.
  • To explore the molecular mechanism underlying HHIP-AS1's role in NSCLC progression.

Main Methods:

  • TCGA database analysis for HHIP-AS1 and HHIP expression prediction.
  • qRT-PCR and Western blotting for expression detection.
  • Cell proliferation, migration, and invasion assays (CCK-8, trans-well).

Main Results:

  • HHIP-AS1 and HHIP expression are downregulated in NSCLC, correlating with poor prognosis.
  • Overexpression of HHIP-AS1 suppresses NSCLC cell proliferation, migration, and invasion.
  • HHIP-AS1 enhances HHIP expression and stability through CELF2 interaction.

Conclusions:

  • HHIP-AS1 acts as a tumor suppressor in NSCLC.
  • HHIP-AS1 restrains NSCLC cell proliferation, migration, and invasion by stabilizing HHIP via CELF2.
  • HHIP-AS1 represents a potential therapeutic target for NSCLC.

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