Recent Advances in CRISPR-Cas Systems for Dengue Virus Detection

Area of Science:

• Molecular Biology
• Infectious Diseases
• Biotechnology

Background:

• Dengue virus (DENV) infections pose a significant global health challenge, with severe dengue leading to high morbidity and mortality.
• Conventional diagnostic methods struggle to keep pace with the escalating DENV burden, necessitating innovative solutions.

Purpose of the Study:

• To review recent advances in CRISPR-Cas technologies for sensitive and robust detection of dengue virus nucleic acids.
• To critically evaluate the advantages, limitations, and future prospects of CRISPR-based diagnostics for DENV.

Main Methods:

• Focus on the roles of Cas12 and Cas13 enzymes in DENV-RNA detection.
• Exploration of field-deployable and portable CRISPR-Cas systems.
• Integration of CRISPR with amplification techniques (hybrid, isothermal), electrochemical sensing, and nanotechnology.

Main Results:

• CRISPR-Cas technologies demonstrate high sensitivity and robustness for DENV nucleic acid detection.
• Serotype-specific detection and portable diagnostic platforms are advancing.
• Hybrid approaches combining CRISPR with other technologies enhance detection capabilities.

Conclusions:

• CRISPR-Cas-based diagnostics represent a next-generation solution for rapid and effective DENV detection.
• These technologies have the potential to significantly improve dengue virus surveillance and control strategies.
• Further development is crucial for widespread clinical and field application.