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Circ_MBNL3 Restrains Hepatocellular Carcinoma Progression by Sponging miR-873-5p to Release PHF2

Lan Peng1, Jian Chen1, Min Li2

  • 1Department of Ultrasound, Wushan County Hospital of Tcm, Chongqing, China.

Biochemical Genetics
|November 15, 2022
PubMed

Insights

Circular RNAs (circRNAs) like circ_MBNL3 are downregulated in hepatocellular carcinoma (HCC). Overexpressing circ_MBNL3 inhibits HCC cell growth and progression by regulating the miR-873-5p/PHF2 pathway.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Circular RNAs (circRNAs) play crucial roles in human cancers.
  • The specific functions of circRNAs in hepatocellular carcinoma (HCC) require further investigation.

Purpose of the Study:

  • To elucidate the role and mechanism of circ_MBNL3 in hepatocellular carcinoma (HCC).

Main Methods:

  • Assessed cell proliferation using MTT, EdU, and colony formation assays.
  • Utilized a xenograft tumor model to evaluate circ_MBNL3's effect on tumor growth in vivo.
  • Investigated the interaction between circ_MBNL3, miR-873-5p, and PHF2 in HCC cells.

Main Results:

  • Circ_MBNL3 expression was significantly decreased in HCC tissues and cell lines.
  • Overexpression of circ_MBNL3 suppressed HCC cell proliferation, migration, and invasion, while promoting apoptosis.
  • Circ_MBNL3 inhibited xenograft tumor growth in vivo.
  • miR-873-5p targeted PHF2, and this interaction was modulated by circ_MBNL3.

Conclusions:

  • Circ_MBNL3 acts as a tumor suppressor in HCC.
  • Circ_MBNL3 restrains HCC progression by regulating the miR-873-5p/PHF2 axis.
  • Circ_MBNL3 holds potential as a therapeutic target for HCC.
Keywords:
Hepatocellular carcinomaPHF2circ_MBNL3miR-873-5p

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