Low Temperature Plasma Jet Affects Acute Skin Wounds in Diabetic Mice Through Reactive Components.
Yang Ge1, Jun Wang1,2, Wei Cao1
1The CAS Key Laboratory of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, Jiangsu, China.
The International Journal of Lower Extremity Wounds
|November 16, 2022
Summary
Low Temperature Plasma Jet (LTPJ) therapy shows promise for diabetic foot ulcers by promoting initial healing and collagen deposition. However, it did not significantly improve overall diabetic wound healing rates in mice.
Area of Science:
- Biomedical Engineering
- Wound Healing Research
- Plasma Medicine
Background:
- Diabetic foot ulcers are a severe complication of diabetes, often leading to poor quality of life and amputation.
- Effective treatments for diabetic foot ulcers remain a critical unmet medical need.
Purpose of the Study:
- To investigate the efficacy of a novel Low Temperature Plasma Jet (LTPJ) system for treating diabetic foot ulcers.
- To analyze the impact of LTPJ on wound healing parameters in both normal and diabetic mice.
Main Methods:
- Development and application of a Low Temperature Plasma Jet (LTPJ) system generating reactive nitrogen and oxygen species (RNOS).
- Treatment of streptozotocin-induced diabetic mice and normal mice with LTPJ.
- Assessment of wound healing through wound area reduction, histological analysis, and immunohistochemistry.
Main Results:
- LTPJ treatment significantly reduced wound area in normal mice.
- Faster collagen deposition and increased vessel formation were observed in plasma-treated normal and diabetic mice on Day 3.
- Diabetic wounds exhibited poor collagen deposition and angiogenesis on Day 8, indicating limited long-term healing improvement.
Conclusions:
- LTPJ improved acute wound healing in normal mice.
- The therapy enhanced initial collagen deposition and angiogenesis in diabetic wounds.
- LTPJ did not significantly accelerate the overall diabetic wound healing rate, possibly due to an imbalance of reactive species.
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