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Related Experiment Video

Updated: Aug 21, 2025

Author Spotlight: Advancements in PBMC Isolation – Enhancing Throughput and Consistency in Research

Author Spotlight: Advancements in PBMC Isolation – Enhancing Throughput and Consistency in Research

Published on: July 19, 2024

Ultrathroughput immunomagnetic cell sorting platform.

David N Philpott¹, Kangfu Chen², Randy S Atwal^2,3

¹Edward S. Rogers Sr. Department of Electrical & Computer Engineering, University of Toronto, Toronto, Ontario, Canada.

|November 16, 2022

Summary

This summary is machine-generated.

We developed a novel microfluidic cell sorter that processes millions of live cells hourly for cell therapies. This ultrathroughput platform rapidly isolates cells for CAR-NK therapies, overcoming current limitations in cell sorting technology.

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Published on: December 22, 2023

Area of Science:

Biotechnology
Cell Biology
Microfluidics

Background:

High-throughput cell sorting is essential for developing cell-based therapies and screening platforms.
Existing cytometry methods face limitations in throughput, cell population isolation, viability, and cost.

Purpose of the Study:

To introduce an ultrathroughput microfluidic cell sorter for high-volume live cell processing.
To demonstrate the platform's capability in isolating clinically relevant cell populations for therapeutic applications.

Main Methods:

Development of a next-generation microfluidic cell sorter (NG-MICS) integrating 3D printing, reversible clamp sealing, and superhydrophobic treatments.
Utilizing protein expression for cell sorting at unprecedented speeds.
Demonstration using peripheral blood mononuclear cells for natural killer cell isolation.

Main Results:

The NG-MICS device achieves ultrathroughput processing of hundreds of millions of live cells per hour.
The platform enables rapid isolation of mature natural killer cells from peripheral blood mononuclear cells.
The developed cell sorter is reusable, user-friendly, and suitable for clinical-scale applications.

Conclusions:

The NG-MICS platform significantly advances cell sorting capabilities, enabling ultrathroughput processing.
This technology facilitates the isolation of specific cell populations, such as those required for CAR-NK therapies.
The device offers a cost-effective and efficient solution for cell-based therapy development and research.