Related Experiment Video
Updated: Aug 21, 2025
Generation of Human Chimeric Antigen Receptor Regulatory T Cells
Published on: January 3, 2025
Generation of non-human primate CAR Tregs using artificial antigen-presenting cells, simian tropic lentiviral
Gavin I Ellis1, Mosha Z Deng1, Delaine W Winn1
1Department of Microbiology and Center for Cellular Immunotherapies, University of Pennsylvania, Philadelphia, PA 19104, USA.
Abstract:
It is technically challenging to generate large doses of regulatory T cells (Tregs) engineered to express a chimeric antigen receptor (CAR) in non-human primates (NHP). Here, we have optimized the manufacturing of CAR Tregs by stringent sorting of Tregs, stimulation by artificial antigen-presenting cells, transduction by simian tropic lentiviral vectors, and antigen-specific expansion. The result of this method is highly suppressive CAR Tregs for use in a pre-clinical, large animal model of transplant tolerance. For complete details on the use and execution of this protocol, please refer to Ellis et al. (2022).
