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¹Google LLC, Mountain, CA 94043, USA.

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Barcode cycle sequencing (BCS) offers a high-throughput method for analyzing molecular binding. This next-generation sequencing (NGS) based assay efficiently identifies binding events between binders and targets using DNA barcodes.

Area of Science:

Biochemistry
Molecular Biology
Genomics

Background:

Characterizing molecular binding properties is crucial but challenging in complex biological systems.
Existing high-throughput assays for binder-target interactions are often cost-prohibitive and limited in scale.

Purpose of the Study:

To introduce and describe Barcode Cycle Sequencing (BCS) as a novel molecular binding assay.
To provide a method for high-throughput characterization of binder-target interactions.

Main Methods:

Barcode Cycle Sequencing (BCS) utilizes a next-generation sequencing (NGS) platform.
Binder libraries and targets are labeled with unique DNA barcodes.
Ligation of binder and target barcodes upon binding allows for sequencing and identification of binding events.

Keywords:

Genomics High throughput screening Molecular biology Sequencing

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Related Experiment Videos

Last Updated: Aug 21, 2025

Visual Detection of Multiple Nucleic Acids in a Capillary Array

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Main Results:

BCS enables large-scale, high-throughput analysis of binding specificities.
The assay is designed for complex environments with numerous potential binder-target pairs.
Encoded binding events are identified through DNA sequencing.

Conclusions:

BCS presents a cost-effective and scalable solution for molecular binding assays.
This method facilitates comprehensive characterization of protein, peptide, and small molecule interactions.
BCS is a valuable tool for advancing research in molecular recognition and drug discovery.