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Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification
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The gelatin-based liquid marbles for cell cryopreservation.

Min Liu1, Changhong Chen1, Jiajun Yu1

  • 1Department of Polymer Science, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China.

Materials Today. Bio
|November 17, 2022
PubMed
Summary

Liquid marbles offer a novel, non-toxic cryopreservation method. Using gelatin, this approach avoids harmful dimethyl sulfoxide (DMSO) and fetal bovine serum (FBS), achieving high cell survival rates.

Keywords:
Cell cryopreservationCryoprotectantGelatinIce recrystallization inhibitionLiquid marble

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Area of Science:

  • Biotechnology
  • Cryobiology
  • Materials Science

Background:

  • Current cell cryopreservation relies on dimethyl sulfoxide (DMSO) and fetal bovine serum (FBS), which have toxicity and contamination risks.
  • Effective cell preservation methods independent of DMSO and FBS are needed for broader applications.

Purpose of the Study:

  • To investigate the potential of liquid marbles as a DMSO- and FBS-free cryopreservation tool.
  • To evaluate the cryoprotective properties of gelatin within a liquid marble system.

Main Methods:

  • Utilized liquid marbles formulated with gelatin solution for cell cryopreservation.
  • Assessed cryopreservation efficiency and cell functionality after freeze-thaw cycles using mouse fibroblast L929 cells.

Main Results:

  • Gelatin-based liquid marbles demonstrated high ice recrystallization inhibition (IRI) and induced smaller ice crystals.
  • Achieved a high cryopreservation efficiency of 90% for L929 cells.
  • Recovered cells maintained normal biological functions after cryopreservation.

Conclusions:

  • Gelatin-based liquid marbles provide an efficient and non-toxic alternative for cell cryopreservation.
  • This method eliminates the need for DMSO and FBS, addressing their limitations in cell preservation.