Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Protocadherin-1 deficiency increases baseline and allergen-induced airway hyperresponsiveness in mice.

American journal of respiratory cell and molecular biology·2026
Same author

Diversion of Arginine's dietary metabolic fate in proteinuric kidney disease.

Research square·2026
Same author

RBMX functional retrocopy safeguards brain development in a species-dependent context.

Brain : a journal of neurology·2026
Same author

The Interplay between Gut Microbiota and Diet-Induced Kidney Protection.

Kidney360·2026
Same author

Novel Netrin-1-Unc5b-Klf4 axis balances AT2 survival in experimental bronchopulmonary dysplasia.

American journal of respiratory cell and molecular biology·2026
Same author

Multifold increase in spinal inhibitory cell types with emergence of limb movement.

Cell reports·2026
Same journal

Evaluation of an open-face 8-channel transmit 64-channel receive 7T head coil for neuroimaging.

Frontiers in neuroscience·2026
Same journal

Acoustic stimulation in pain management: neurobiological mechanisms and clinical applications-a narrative review.

Frontiers in neuroscience·2026
Same journal

Local brain connectome parameters across the spectrum of clinical cognitive decline.

Frontiers in neuroscience·2026
Same journal

Body mass index affects EEG microstate dynamics through blood viscosity in high-altitude environments.

Frontiers in neuroscience·2026
Same journal

Disrupted glymphatic function and its relationship with sleep and cognitive impairment in ME/CFS assessed via DTI-ALPS.

Frontiers in neuroscience·2026
Same journal

Neuromorphic-inspired multi-view global-local fusion for IR-UWB radar dynamic gesture recognition.

Frontiers in neuroscience·2026
See all related articles

Related Experiment Video

Updated: Aug 21, 2025

Ex Utero Electroporation and Organotypic Slice Cultures of Embryonic Mouse Brains for Live-Imaging of Migrating GABAergic Interneurons
09:50

Ex Utero Electroporation and Organotypic Slice Cultures of Embryonic Mouse Brains for Live-Imaging of Migrating GABAergic Interneurons

Published on: April 20, 2018

10.1K

Modifying PCDH19 levels affects cortical interneuron migration.

Anna Pancho1, Manuela D Mitsogiannis1, Tania Aerts1

  • 1Developmental Neurobiology Group, Animal Physiology and Neurobiology Division, Department of Biology, KU Leuven, Leuven, Belgium.

Frontiers in Neuroscience
|November 17, 2022
PubMed
Summary
This summary is machine-generated.

Altering PCDH19 protein dosage impacts cortical interneuron migration during development. This suggests PCDH19

Keywords:
PCDH19-CEbrain developmentinterneuronmedial ganglionic eminenceneurodevelopmental disorderneuronal migration

More Related Videos

Transplantation of Chemogenetically Engineered Cortical Interneuron Progenitors into Early Postnatal Mouse Brains
06:09

Transplantation of Chemogenetically Engineered Cortical Interneuron Progenitors into Early Postnatal Mouse Brains

Published on: August 26, 2019

6.0K
A Novel Strategy Combining Array-CGH, Whole-exome Sequencing and In Utero Electroporation in Rodents to Identify Causative Genes for Brain Malformations
08:22

A Novel Strategy Combining Array-CGH, Whole-exome Sequencing and In Utero Electroporation in Rodents to Identify Causative Genes for Brain Malformations

Published on: December 1, 2017

8.7K

Related Experiment Videos

Last Updated: Aug 21, 2025

Ex Utero Electroporation and Organotypic Slice Cultures of Embryonic Mouse Brains for Live-Imaging of Migrating GABAergic Interneurons
09:50

Ex Utero Electroporation and Organotypic Slice Cultures of Embryonic Mouse Brains for Live-Imaging of Migrating GABAergic Interneurons

Published on: April 20, 2018

10.1K
Transplantation of Chemogenetically Engineered Cortical Interneuron Progenitors into Early Postnatal Mouse Brains
06:09

Transplantation of Chemogenetically Engineered Cortical Interneuron Progenitors into Early Postnatal Mouse Brains

Published on: August 26, 2019

6.0K
A Novel Strategy Combining Array-CGH, Whole-exome Sequencing and In Utero Electroporation in Rodents to Identify Causative Genes for Brain Malformations
08:22

A Novel Strategy Combining Array-CGH, Whole-exome Sequencing and In Utero Electroporation in Rodents to Identify Causative Genes for Brain Malformations

Published on: December 1, 2017

8.7K

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Genetics

Background:

  • PCDH19 protein, encoded by the X-chromosome, is implicated in PCDH19-clustering epilepsy (PCDH19-CE) due to mutations.
  • Disturbed cell-cell contacts from mosaic PCDH19 absence are hypothesized to cause PCDH19-CE.
  • Previous research focused on PCDH19's role in cortical and hippocampal neurons, not interneurons.

Purpose of the Study:

  • Investigate PCDH19's role in cortical interneuron migration during embryogenesis.
  • Determine the impact of PCDH19 dosage on interneuron migration.
  • Explore domain-specific functions of PCDH19 in neuronal development.

Main Methods:

  • Overexpression and reduced expression of PCDH19 in brain slices and medial ganglionic eminence (MGE) explants.
  • In vivo studies using heterozygote and homozygote PCDH19 knockout mouse models.
  • Analysis of cell survival and migration patterns, including non-cell-autonomous effects.

Main Results:

  • Altering PCDH19 dosage, via overexpression or reduction, affects cortical interneuron migration.
  • Subtle defects in interneuron migration were observed with reduced PCDH19 expression.
  • In vivo studies showed mild interneuron migration reduction in heterozygote knockout animals.
  • Domain-specific effects of PCDH19 on cell survival and migration were identified.
  • Non-cell-autonomous effects of PCDH19 dosage changes on neighboring cells were observed.

Conclusions:

  • Cortical interneuron migration is sensitive to PCDH19 dosage during embryogenesis.
  • Both extracellular and cytoplasmic domains of PCDH19 play significant roles.
  • Balanced PCDH19 dosage is crucial for proper cortical interneuron migration.
  • Findings suggest novel roles for PCDH19 in neurodevelopmental processes relevant to epilepsy.