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Related Experiment Video

Updated: Aug 20, 2025

Microfluidic Chip Fabrication and Method to Detect Influenza
09:43

Microfluidic Chip Fabrication and Method to Detect Influenza

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Integrated microdroplet array platform with temperature controller and micro-stirring for ultra-fast SARS-CoV-2

Mengyun Zhou1, Yong Luo1, Lirong Wang1

  • 1Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, School of Biomedical Engineering, Health Science Center, Shenzhen University, Shenzhen, Guangdong, 518060, PR China.

Biosensors & Bioelectronics
|November 17, 2022
PubMed
Summary

A new portable microdroplet platform offers rapid COVID-19 diagnosis. This point-of-care test (POCT) uses precise temperature control and micro-stirring for ultrafast results, aiding infectious disease detection.

Keywords:
Enhanced RT-LAMPIntegratedSARS-CoV-2 detectionTemperature controllerUltrasound

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Area of Science:

  • Biotechnology
  • Medical Diagnostics
  • Point-of-Care Testing

Background:

  • COVID-19 presents a significant global health challenge.
  • Rapid and sensitive point-of-care testing (POCT) is crucial for controlling outbreaks.
  • Existing diagnostic methods may lack speed and portability for in-situ deployment.

Purpose of the Study:

  • To develop a portable, high-throughput microdroplet diagnostic platform for SARS-CoV-2.
  • To achieve ultrafast and sensitive detection of COVID-19 at the point of care.
  • To create a versatile tool for infectious disease diagnosis in resource-limited settings.

Main Methods:

  • Development of a portable microdroplet detection platform with integrated temperature control and micro-stirring.
  • Utilizing a p-n junction for precise, independent temperature control within microdroplets for reverse transcription loop-mediated isothermal amplification (RT-LAMP).
  • Incorporating an ultrasonic micro-stirring unit to enhance reaction kinetics.

Main Results:

  • Demonstrated good linearity over a wide range (1 to 10^5 copies/μL) with a low limit of detection (LOD) of 0.48 copy/μL.
  • Achieved reporting times as fast as 6.1 minutes for high-viral load samples.
  • Completed the total detection process, including sample preparation, within 30 minutes.

Conclusions:

  • The developed portable microdroplet platform enables high-throughput and ultrafast COVID-19 diagnosis.
  • The integrated p-n junction temperature controller and ultrasonic micro-stirring enhance RT-LAMP efficiency.
  • This fully integrated molecular diagnostic platform is a promising tool for point-of-care infectious disease detection, especially in resource-limited environments.