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Updated: Jul 17, 2026

Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography
Published on: February 9, 2019
Quantitative dynamic full-field optical coherence tomography for high-resolution intraoperative imaging of head and
Zixuan Huang1, Mengyun Zhou2, Zhongzhou Luo2
1Department of Otolaryngology, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou 510655, China.
Abstract:
We present a dynamic full-field optical coherence tomography (D-FFOCT) system providing both structural and dynamic contrast for label-free imaging of head and neck squamous cell carcinoma (HNSCC). The system simultaneously acquires structural and dynamic contrast, enabling visualization of tissue morphology and intracellular activity. Ex vivo imaging of MTCQ1 xenografts and fresh human HNSCC specimens showed distinct patterns between tumor and normal tissues, consistent with H&E histology. Dynamic contrast remained stable for 4 h after excision. Quantitative ROI analysis revealed significant differences in epithelial morphology and temporal fluctuation metrics, supporting rapid intraoperative optical characterization of HNSCC.
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