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Updated: Aug 20, 2025

Chromatin Immunoprecipitation of Murine Brown Adipose Tissue
Published on: November 21, 2018
A proposed metric set for evaluation of genome assembly quality
1Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture and Rural Affairs, Institute of Tropical Crop Genetic Resources, Chinese Academy of Tropical Agricultural Sciences, No. 4 Xueyuan Rd, Haikou City, Hainan 571101, China.
Abstract:
Quality control is essential for genome assemblies; however, a consensus has yet to be reached on what metrics should be adopted for the evaluation of assembly quality. N50 is widely used for contiguity measurement, but its effectiveness is constantly in question. Prevailing metrics for the completeness evaluation focus on gene space, yet challenging areas such as tandem repeats are commonly overlooked. Achieving correctness has become an indispensable dimension for quality control, while prevailing assembly releases lack scores reflecting this aspect. We propose a metric set with a set of statistic indexes for effective, comprehensive evaluation of assemblies and provide a score of a finished assembly for each metric, which can be utilized as a benchmark for achieving high-quality genome assemblies.
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