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Updated: Aug 20, 2025

Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification
Published on: March 31, 2021
SARS-CoV-2 variants of concern surveillance including Omicron using RT-PCR-based genotyping offers comparable
Simone Vanoni1, Arnoldas Matulevicius1, Besard Avdiu1
1PharmGenetix GmbH, Niederalm/Anif, Austria.
Abstract:
Known SARS-CoV-2 variants of concern (VOCs) can be detected and differentiated using an RT-PCR-based genotyping approach, which offers quicker time to result, lower cost, higher flexibility, and use of the same laboratory instrumentation for detection of SARS-CoV-2 when compared with whole genome sequencing (WGS). In the current study, we demonstrate how we applied a genotyping approach for identification of all VOCs and that such technique can offer comparable performance to WGS for identification of known SARS-CoV-2 VOCs, including more recent strains, Omicron BA.1 and BA.2.
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