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Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

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Multiple-Streams Focusing-Based Cell Separation in High Viscoelasticity Flow.

Haidong Feng^1,2, Dhruv Patel¹, Jules J Magda³

¹Department of Mechanical Engineering, University of Utah, Salt Lake City, Utah84112, United States.

|November 21, 2022

Summary

This summary is machine-generated.

High viscoelastic flow enables multiple-stream focusing for cell separation. This method separates cells by size and deformability, including malaria-infected red blood cells, advancing biofluid sample treatment.

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Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

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Area of Science:

Biophysics
Microfluidics
Cellular Biology

Background:

Viscoelastic flow is utilized in microfluidic particle separation.
Particles typically focus at the channel center in diluted viscoelastic flow.

Purpose of the Study:

To observe and apply the transition from single-stream to multiple-streams focusing (MSF) in high viscoelastic flow for cell separation.
To investigate the influence of cell physical properties on MSF formation.
To demonstrate cell separation based on size and deformability.

Main Methods:

Observation of particle focusing stream bifurcation in high viscoelastic flow.
Analysis of the balance between elastic force and viscoelastic secondary flow drag force.
Studying the impact of cell dimension, shape, and deformability on MSF.

Main Results:

Transition from single-stream to multiple-streams focusing observed in high viscoelastic flow.
Particle separation achieved based on size (RBC vs. WBC) and deformability.
Demonstrated separation of malaria-infected red blood cells based on altered deformability.

Conclusions:

The study elucidates particle movement dynamics in high viscoelastic flow.
Multiple-streams focusing offers novel particle manipulation and separation strategies.
This technique provides new avenues for biofluid sample treatment.