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Updated: Aug 20, 2025

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms
Published on: February 2, 2024
Computational approaches and challenges for identification and annotation of non-coding RNAs using RNA-Seq
Kiran Dindhoria1, Isha Monga2, Amarinder Singh Thind3,4
1Institute of Microbial Technology, Council of Scientific and Industrial Research, Chandigarh, India.
Abstract:
Significant innovations in next-generation sequencing techniques and bioinformatics tools have impacted our appreciation and understanding of RNA. Practical RNASeq applications have evolved in conjunction with sequence technology and bioinformatic tool advances. In this review, we explained various computational resources, tools, and bioinformatics analyses advancement for small and large non-coding RNAs. These include non-coding RNAs (ncRNAs) such as piwi, micro, circular, and long ncRNAs. In addition, this article discusses future challenges, single-cell level sequencing for non-coding RNAs, and advantages of using long-read sequencing to annotate lncRNAs.
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