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Human lung cell models to study aerosol delivery - considerations for model design and development
Barbara Rothen-Rutishauser1, Matthew Gibb2, Ruiwen He1
1BioNanomaterials, Adolphe Merkle Institute, University Fribourg, Chemin des Verdiers 4 CH-1700, Fribourg, Switzerland.
Summary
This review guides the design of human lung tissue models for aerosol research. It covers scaffold choice, cell types, culture conditions, exposure methods, and verification for accurate studies.
Area of Science:
- Biomedical Engineering
- Respiratory Physiology
- In Vitro Modeling
Background:
- Human lung tissue models are crucial for studying aerosol interactions and their effects.
- Existing models vary from simple cell cultures to complex 3D co-cultures.
- Model selection depends on specific research scenarios, adverse outcomes, and endpoints.
Approach:
- This review synthesizes functional and structural aspects of human lung tissue.
- It details key considerations for designing lung tissue models.
- Focuses on scaffold selection, cell types, culture conditions, aerosol exposure, and verification.
Key Points:
- Appropriate scaffold selection is vital for mimicking lung architecture.
- Choosing relevant cell types (healthy and diseased) enhances model fidelity.
- Optimized culture conditions and assembly are critical for physiological relevance.
- Aerosol exposure methods must align with intended research scenarios.
- Defined endpoints and verification processes ensure model reliability.
Conclusions:
- Effective lung tissue model design requires careful consideration of multiple factors.
- Advanced models are essential for accurate prediction of aerosol behavior in the respiratory system.
- Further research is needed to address remaining challenges and advance the field.
