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Updated: Aug 20, 2025

A Cryo-pulverization Protocol for Processing Mouse Paws to Evaluate Molecular Pathways of Tissue Inflammation in a Collagen Induced Arthritis Model
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A Cryo-pulverization Protocol for Processing Mouse Paws to Evaluate Molecular Pathways of Tissue Inflammation in a Collagen Induced Arthritis Model

Published on: October 30, 2019

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Expression and function analysis of microRNA in collagen-induced arthritis rats

Yuqi Wen1, Cailin He2, Yang Wang2

  • 1Department of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha 410008. 208111147@csu.edu.cn.

Zhong Nan Da Xue Xue Bao. Yi Xue Ban = Journal of Central South University. Medical Sciences
|November 22, 2022
PubMed
Abstract

Insights

This study identified 69 differentially expressed microRNAs (miRNAs) in a rat model of rheumatoid arthritis. These miRNAs are linked to cellular metabolism and signaling pathways like MAPK and Wnt.

Area of Science:

  • Molecular Biology
  • Immunology
  • Genetics

Background:

  • Rheumatoid arthritis (RA) is a prevalent autoimmune disease.
  • MicroRNAs (miRNAs) play a role in the pathogenesis of RA.

Purpose of the Study:

  • To identify differentially expressed miRNAs in collagen-induced arthritis (CIA) rats.
  • To analyze the biological functions and pathways of miRNA target genes.

Main Methods:

  • RNA extraction from synovium of experimental animals.
  • miRNA gene profiling using miRNA microarray.
  • Prediction of target mRNAs and enrichment analysis (GO, KEGG).

Main Results:

  • 69 differentially expressed miRNAs were found in CIA rats (22 up-regulated, 47 down-regulated).
  • Up-regulated miRNA targets are enriched in cellular metabolism and MAPK/Wnt signaling.
  • Down-regulated miRNA targets are enriched in nervous system development and axon guidance pathways.

Conclusions:

  • Differentially expressed miRNAs are present in the CIA rat model.
  • These miRNAs may influence metabolic functions and signaling pathways (MAPK, Wnt).
Keywords:
collagen-induced arthritis ratsmicroRNArheumatoid arthritis

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