Related Experiment Video
Updated: Aug 20, 2025

Assaying Proteasomal Degradation in a Cell-free System in Plants
Published on: March 26, 2014
Detection and Quantification of Protein Aggregates in Plants
Ujjal Jyoti Phukan1, Simon Stael2,3, Amanda Gonçalves4,5
1Centre for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UB, Campus UAB, Barcelona, Spain.
Abstract:
Protein quality control is an important aspect of stress recovery. It maintains protein homeostasis through a machinery of regulatory proteins such as chaperones and proteases. When the system recognizes accumulation of misfolded or aggregated proteins, the cell recruits a set of regulatory proteins to initiate protein quality control. To understand the dynamics of stress-mediated aggregate protein formation and recovery in plants, robust methods aimed at detecting and measuring such protein aggregates are needed. This will help us to deepen our understanding of protein quality control mechanisms in plants.

