Related Experiment Video
Updated: Aug 20, 2025

Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics
Published on: October 13, 2020
Peptidoglycan NlpC/P60 peptidases in bacterial physiology and host interactions
Matthew E Griffin1, Steven Klupt1, Juliel Espinosa2
1Department of Immunology and Microbiology, Scripps Research, La Jolla, CA 92037, USA.
Abstract:
The bacterial cell wall is composed of a highly crosslinked matrix of glycopeptide polymers known as peptidoglycan that dictates bacterial cell morphology and protects against environmental stresses. Regulation of peptidoglycan turnover is therefore crucial for bacterial survival and growth and is mediated by key protein complexes and enzyme families. Here, we review the prevalence, structure, and activity of NlpC/P60 peptidases, a family of peptidoglycan hydrolases that are crucial for cell wall turnover and division as well as interactions with antibiotics and different hosts. Understanding the molecular functions of NlpC/P60 peptidases should provide important insight into bacterial physiology, their interactions with different kingdoms of life, and the development of new therapeutic approaches.
More Related Videos
Related Concept Videos
Peptidoglycan Synthesis
Formation of Lipopolysaccharides
Bacterial Cell Wall
Outer Layers of the Cell Envelope
Cytoskeletal Proteins in Bacteria
Gene Regulation in Microbial Communities: Quorum Sensing

