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LINC01311 exerts an inhibitory effect in thyroid cancer progression by targeting the miR-146b-5p/IMPA2 axis

Mengjiang Liu1, Linghui Zhang1, Juping Hu1

  • 1Department of Endocrinology, Hubei NO.3 People's Hospital of Jianghan University, No. 26, Zhongshan Avenue, Qiaokou District, Wuhan, Hubei 430033, China.

Translational Oncology
|November 24, 2022
PubMed
Abstract

Insights

Long non-coding RNA LINC01311 inhibits thyroid cancer (TC) progression by targeting the miR-146b-5p/IMPA2 pathway. Targeting this axis offers a potential therapeutic strategy for TC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long non-coding RNAs (lncRNAs) are implicated in cancer development, including thyroid cancer (TC).
  • The specific role and regulatory mechanisms of LINC01311 in TC remain to be fully elucidated.

Purpose of the Study:

  • To investigate the function and molecular mechanisms of LINC01311 in thyroid cancer.
  • To explore the potential of targeting the LINC01311/miR-146b-5p/IMPA2 axis for TC treatment.

Main Methods:

  • Quantitative analysis of LINC01311, miR-146b-5p, and IMPA2 expression using RT-qPCR and western blotting.
  • In vitro assays (CCK-8, scratch-wound healing, flow cytometry) and in vivo xenograft models to assess proliferation, migration, and apoptosis.
  • Luciferase reporter and RNA immunoprecipitation (RIP) assays to confirm molecular interactions.

Main Results:

  • LINC01311 and IMPA2 were downregulated, while miR-146b-5p was upregulated in TC tissues and cells.
  • LINC01311 overexpression suppressed TC cell proliferation and migration in vitro and tumor growth in vivo.
  • miR-146b-5p directly targets both LINC01311 and IMPA2, and IMPA2 upregulation can rescue the inhibitory effects of miR-146b-5p.

Conclusions:

  • LINC01311 inhibits TC cell growth by regulating the miR-146b-5p/IMPA2 axis.
  • The LINC01311/miR-146b-5p/IMPA2 pathway represents a potential therapeutic target for managing thyroid cancer progression.
Keywords:
IMPA2LINC01311MigrationProliferationThyroid cancermiR-146b-5p

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