Time-Resolved Persistent Luminescence Encoding for Multiplexed Severe Acute Respiratory Syndrome Coronavirus 2...

Area of Science:

• Biomedical Diagnostics
• Optical Biosensing
• Nanotechnology

Background:

• Accurate diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) requires precise optical multiplexing.
• Spectral crowding and background interference are significant challenges in current diagnostic methods.

Purpose of the Study:

• To develop a novel persistent luminescence (PersL) lifetime/color binary encoding strategy for SARS-CoV-2 diagnosis.
• To overcome limitations of spectral crowding and background interference in optical multiplexing.

Main Methods:

• Utilized luminescence resonance energy transfer (LRET) processes to manipulate PersL nanoplatforms.
• Developed a temporal coding dimension using PersL lifetimes and emissions across three channels.
• Employed time-resolved PersL technology for decoding barcoding populations.
• Functionalized PersL nanoplatforms for simultaneous quantification of five-plex SARS-CoV-2 biomarkers.

Main Results:

• Achieved precise simultaneous multitarget identification within a minimized sample.
• Demonstrated autofluorescence-free background and high-throughput capability.
• Successfully decoded at least four populations of barcoding in a single channel.
• Attained limits of detection in the subnanomolar range for five-plex SARS-CoV-2 biomarkers.
• Enabled highly differentiable discrimination of multitargets at ultralow background concentrations.

Conclusions:

• The proposed PersL binary encoding strategy provides a powerful tool for optical multiplexing in SARS-CoV-2 diagnosis.
• This approach offers high-fidelity resolution and advanced capabilities for biomedical applications.
• PersL nanoplatforms significantly advance multiplexed detection technologies.