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Ufana Riaz1,2, Nuzhat Nabi1, India Pointer2

  • 1Materials Research Laboratory, Department of Chemistry, Jamia Millia Islamia New Delhi-110025 India uriaz@jmi.ac.in ufana2002@yahoo.co.in.

RSC Advances
|November 25, 2022
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Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.