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Related Experiment Video

Updated: Aug 20, 2025

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

Instant three-color multiplane fluorescence microscopy.

Ingo Gregor¹, Eugenia Butkevich¹, Jörg Enderlein^1,2

¹III. Institute of Physics - Biophysics.

Biophysical Reports

|November 25, 2022

Summary

This summary is machine-generated.

Researchers developed a new fluorescence microscope for faster, high-resolution live-cell imaging. This multiplane, multicolor system captures volumetric data rapidly, overcoming limitations of current technologies for biological research.

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Last Updated: Aug 20, 2025

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

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Published on: December 9, 2013

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

Area of Science:

Microscopy
Cell Biology
Biomedical Imaging

Background:

Fluorescence microscopy is crucial in biology and medicine due to its specificity and sensitivity.
Live-cell imaging demands high spatial resolution, rapid acquisition, 3D sectioning, and multicolor detection.
Existing microscopes often compromise on these essential live-cell imaging requirements.

Purpose of the Study:

To present a novel multiplane, multicolor wide-field microscope.
To overcome the limitations of current fluorescence microscopes in live-cell imaging.
To enable high-speed volumetric data acquisition for biological samples.

Main Methods:

Development of a multiplane, multicolor wide-field microscope using a dedicated beam splitter.
Recording volumetric data across eight focal planes and three emission colors.
Utilizing commercially available components for straightforward implementation.

Main Results:

Achieved frame rates of hundreds of volumes per second.
Demonstrated efficient and high-performance three-dimensional imaging of multiply labeled cells (fixed and living).
The system successfully captures detailed volumetric data rapidly.

Conclusions:

The developed microscope offers a solution for high-speed, multicolor, 3D live-cell imaging.
Its design overcomes compromises inherent in existing fluorescence microscopy systems.
The use of standard components facilitates widespread adoption and application in biological research.