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Updated: Aug 20, 2025

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
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Selective plane illumination microscope dedicated to volumetric imaging in microfluidic chambers.

Caroline Bissardon1, Xavier Mermet1, Clément Quintard1

  • 1Univ. Grenoble Alpes, CEA, LETI, DTBS, F-38000 Grenoble, France.

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Summary

We developed a new microscope for 3D imaging of organ-on-chip models. This selective plane illumination fluorescence microscope offers a large field of view for cellular-scale analysis within microfluidic devices.

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Area of Science:

  • Biomedical Engineering
  • Microscopy
  • Cell Biology

Background:

  • Organ-on-chip technology requires advanced imaging for cellular analysis.
  • Existing microscopy techniques may struggle with volumetric imaging within microfluidic devices.

Purpose of the Study:

  • To present an original selective plane illumination fluorescence microscope.
  • To enable morphological analysis of "Organ-on-chip" biostructures at the cellular scale.

Main Methods:

  • A custom-built selective plane illumination fluorescence microscope.
  • Microscope designed around microfluidic chips for in-situ 3D imaging.
  • Horizontal sample orientation to preserve microfluidic phenomena.

Main Results:

  • The setup achieves a field of view of approximately 200 µm with a resolution of approximately 5 µm.
  • Demonstrated 3D imaging capabilities within microfluidic chambers.
  • Successful imaging of organ-on-chip-like biostructures.

Conclusions:

  • The developed microscope provides a viable solution for 3D imaging of organ-on-chip models.
  • The simple, integrated design facilitates imaging without disturbing microfluidic processes.
  • Enables detailed morphological analysis of cellular structures in microfluidic environments.