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Related Experiment Video

Updated: Apr 1, 2026

Author Spotlight: Improving Reproducibility in Vascular Organoids Using ROCK Inhibitors and Microwell Confinement
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Using vascularized organoids-on-chip for assessing systemic drug delivery.

Emily Tubbs1, Fabrice P Navarro2, Yves Fouillet2

  • 1University Grenoble Alpes, CEA, Inserm, IRIG, UA13 BGE, Biomics, Grenoble, France.

Methods in Cell Biology
|March 30, 2026
PubMed
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This study details a protocol for vascularizing organoids-on-chip, specifically pancreatic islet spheroids. This advancement aids in modeling autoimmune diseases and inflammation for drug development.

Area of Science:

  • Biomedical Engineering
  • Organoid Technology
  • Disease Modeling

Background:

  • Organoids-on-chip offer advanced platforms for drug assessment and disease modeling.
  • Vascularization is crucial for mimicking systemic drug delivery and complex biological processes.
  • Pancreatic islet spheroids are key models for studying diabetes and autoimmune conditions.

Purpose of the Study:

  • To provide a detailed experimental protocol for vascularizing organoids-on-chip.
  • To establish a model for autoimmune diseases using pancreatic islet spheroids.
  • To facilitate research in inflammation, immunotherapies, and drug development.

Main Methods:

  • Development of a hydrogel preparation technique for supporting pancreatic islet spheroids.
  • Implementation of a protocol to induce vascularization within the organoid-on-chip system.
Keywords:
Organoids-on-chipPancreatic islet spheroidsVascularization

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  • Optimization of the organoids-on-chip model for disease simulation.

    • Main Results:

    • Successful vascularization of pancreatic islet spheroids within the organoids-on-chip platform.
    • Demonstration of the model's utility in simulating aspects of autoimmune diseases and inflammation.
    • Establishment of a reproducible protocol for researchers.

    Conclusions:

    • The developed protocol enables the creation of vascularized organoids-on-chip for pancreatic islet studies.
    • This model serves as a valuable tool for investigating autoimmune diseases, inflammation, and immunotherapies.
    • The platform supports drug screening, toxicity testing, and pharmacokinetic/pharmacodynamic studies.