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Updated: Jun 14, 2026

10:57
Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules
Published on: November 2, 2009
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Enzyme self-label-bound ATTO700 in single-molecule and super-resolution microscopy
Michael Trumpp1,2, Anna Oliveras3, Hannes Gonschior1
1Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), Robert-Rössle-Str. 10, 13125 Berlin, Germany. broichhagen@fmp-berlin.de.
Abstract
None:
Herein, we evaluate near-infrared ATTO700 as an acceptor in SNAP- and Halo-tag protein labelling for Förster Resonance Energy Transfer (FRET) by ensemble and single molecule measurements. Microscopy of cell surface proteins in live cells is perfomed including super-resolution stimulated emission by depletion (STED) nanoscopy.

