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Related Experiment Video

Updated: Aug 20, 2025

Author Spotlight: EasyFlow - An Economical and Adaptable Perfusion Bioreactor for Large Blood Vessel Culture
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Mini- and macro-scale direct perfusion bioreactors with optimized flow for engineering 3D tissues.

Gordian Born1, Evelia Plantier2, Guido Nannini3

  • 1Department of Biomedical Engineering, University of Basel, Basel, Switzerland.

Biotechnology Journal
|November 25, 2022
PubMed
Summary

Optimized perfusion bioreactors (Flopper systems) enhance homogenous flow for tissue engineering. Miniaturized versions (mini-Flopper) enable medium-throughput drug testing with limited biological material.

Keywords:
bioreactorperfusion flowtissue engineering

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Area of Science:

  • Tissue Engineering
  • Bioreactor Design
  • Biomedical Engineering

Background:

  • Perfusion bioreactors are crucial for uniform cell distribution and tissue development in tissue engineering.
  • Previous U-shaped perfusion bioreactors (U-CUP) exhibited non-uniform flow, particularly in peripheral scaffold regions.
  • Optimizing flow homogeneity is key to improving engineered tissue quality.

Purpose of the Study:

  • To optimize bioreactor design for homogenous perfusion flow through 3D scaffolds.
  • To miniaturize the optimized macro-scale bioreactor (macro-Flopper) into a mini-scale device (mini-Flopper) for medium-throughput applications.
  • To evaluate the functionality of the Flopper systems in engineering stromal tissues.

Main Methods:

  • Computational fluid dynamic (CFD) modeling was used to analyze flow patterns within the optimized perfusion chamber design.
  • The macro-scale Flopper bioreactor was miniaturized to create the mini-Flopper system.
  • Proof-of-principle studies involved engineering endothelialized stromal tissues using human adipose tissue-derived stromal vascular fraction (SVF) cells.

Main Results:

  • The optimized Flopper bioreactor design demonstrated highly homogenous flow speed, pressure, and shear stress distribution within the scaffold.
  • CFD modeling confirmed improved flow homogeneity compared to previous designs.
  • Successful engineering of endothelialized stromal tissues was achieved using the Flopper systems.

Conclusions:

  • Two Flopper bioreactor models (macro and mini) with optimized perfusion flow and complementary sizes have been developed.
  • These systems show potential for engineering homogenous tissues and for drug testing assays using minimal biological material.
  • Further studies are needed to confirm the benefits of flow optimization on cell maintenance in engineered tissues.