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KRAS Affects the Lipid Composition by Regulating Mitochondrial Functions and MAPK Activation in Bovine Mammary

Wenjie Yu1, Hao Jiang1,2, Fengjiao Liu1

  • 1Department of Laboratory Animals, Jilin Provincial Key Laboratory of Animal Model, Jilin University, Changchun 130062, China.

Animals : an Open Access Journal From MDPI
|November 26, 2022
PubMed

Insights

Inhibition of Kirsten rat sarcoma viral oncogene homolog (KRAS) impacts bovine mammary cell lipid composition and mitochondrial function. This research offers insights into milk fat synthesis and secretion at a molecular level.

Area of Science:

  • Molecular Biology
  • Cellular Metabolism
  • Dairy Science

Background:

  • Kirsten rat sarcoma viral oncogene homolog (KRAS) is a proto-oncogene involved in cell signaling.
  • KRAS's role in milk fat synthesis in bovine mammary epithelial cells is not well understood.
  • Previous research links KRAS to cytokine secretion, cell chemotaxis, and survival.

Purpose of the Study:

  • To investigate the effects of KRAS inhibition on bovine mammary epithelial cells (MAC-T).
  • To determine KRAS's influence on cell metabolism, autophagy, oxidative stress, ER stress, mitochondrial function, and lipid composition.
  • To elucidate the molecular mechanisms underlying KRAS's role in milk fat synthesis.

Main Methods:

  • Utilized an immortalized dairy cow mammary epithelial cell line (MAC-T).
  • Inhibited KRAS expression to observe cellular changes.
  • Analyzed effects on cell metabolism, autophagy, oxidative stress, endoplasmic reticulum stress, mitochondrial function, and lipid composition.

Main Results:

  • KRAS inhibition altered lipid composition, particularly triglyceride levels, in MAC-T cells.
  • Observed changes in mitochondrial function, autophagy, and endoplasmic reticulum stress following KRAS inhibition.
  • KRAS inhibition modulated the activation of mammalian target of rapamycin (mTOR) and mitogen-activated protein kinase (MAPK) pathways.

Conclusions:

  • Regulation of KRAS significantly affects milk fat synthesis and composition in bovine mammary cells.
  • Findings provide a molecular understanding of milk fat production and secretion.
  • Results offer a theoretical basis for improving milk fat percentage and yield in dairy cows.
Keywords:
KRASMAPKbovine mammary epithelial cellendoplasmic reticulumlipid metabolismmilk fattriglyceride

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