Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Public Knowledge, Attitudes, and Perceptions of Antimicrobial Resistance in Brazil: Insights from a Nationwide Online Survey.

Antibiotics (Basel, Switzerland)·2026
Same author

Comparison of digital PCR and real time PCR methods for quantitative analysis of African Swine Fever Virus.

Frontiers in veterinary science·2025
Same author

Antimicrobial Resistance in Chicken Meat: Comparing <i>Salmonella</i>, <i>Escherichia coli</i>, and <i>Enterococcus</i> from Conventional and Antibiotic-Free Productions.

Microorganisms·2025
Same author

Blue Crab (<i>Callinectes sapidus</i>) Haemolymph as a Potential Reservoir of Mesophilic <i>Shewanella</i> Species.

Animals : an open access journal from MDPI·2025
Same author

Microplastics in Mussels (<i>Mytilus galloprovincialis</i>): Understanding Pollution in Italian Seas.

Toxics·2025
Same author

Seasonal variability of trace elements bioaccumulation in Pacific Oysters (Crassostrea gigas) from an experimental pilot farm in the Calich Lagoon (Sardinia, Italy).

Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS)·2024

Related Experiment Video

Updated: Aug 20, 2025

DNA-based Fish Species Identification Protocol
09:15

DNA-based Fish Species Identification Protocol

Published on: April 28, 2010

29.6K

Detection of Fish Allergens in Foods Using an In-House Real-Time PCR Targeting the Ribosomal 18S rRNA Gene.

Simona Cau1, Cinzia Daga1, Carlo Spanu2

  • 1Veterinary Public Health Institute of Sardinia, Complex Structure of Food Hygiene, Via Duca degli Abruzzi 8, 07100 Sassari, Italy.

Foods (Basel, Switzerland)
|November 26, 2022
PubMed
Summary

A new real-time PCR method accurately detects fish DNA in foods, ensuring accurate allergen labeling. This reliable method helps verify compliance with food safety regulations for fish allergens.

Keywords:
absolute detectionfish allergyfood labellingrelative detectionribosomal 18S rRNA

More Related Videos

Improved Polymerase Chain Reaction-restriction Fragment Length Polymorphism Genotyping of Toxic Pufferfish by Liquid Chromatography/Mass Spectrometry
09:34

Improved Polymerase Chain Reaction-restriction Fragment Length Polymorphism Genotyping of Toxic Pufferfish by Liquid Chromatography/Mass Spectrometry

Published on: September 20, 2016

11.2K
Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis
10:33

Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis

Published on: June 17, 2019

10.9K

Related Experiment Videos

Last Updated: Aug 20, 2025

DNA-based Fish Species Identification Protocol
09:15

DNA-based Fish Species Identification Protocol

Published on: April 28, 2010

29.6K
Improved Polymerase Chain Reaction-restriction Fragment Length Polymorphism Genotyping of Toxic Pufferfish by Liquid Chromatography/Mass Spectrometry
09:34

Improved Polymerase Chain Reaction-restriction Fragment Length Polymorphism Genotyping of Toxic Pufferfish by Liquid Chromatography/Mass Spectrometry

Published on: September 20, 2016

11.2K
Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis
10:33

Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis

Published on: June 17, 2019

10.9K

Area of Science:

  • Food Science
  • Molecular Biology
  • Analytical Chemistry

Background:

  • Fish is a common food allergen, posing risks to sensitized individuals.
  • Accurate food labeling is crucial for consumer health and safety.
  • Existing detection methods may lack sensitivity or specificity for trace amounts of fish.

Purpose of the Study:

  • To validate an in-house real-time PCR method for detecting fish DNA in food products.
  • To assess the specificity and sensitivity of the developed method.
  • To evaluate the method's suitability for routine allergen monitoring and regulatory compliance.

Main Methods:

  • Developed a real-time PCR assay targeting the 18S rRNA gene for universal fish DNA detection.
  • Tested primer specificity against various fish, mollusc, crustacean, and other food species.
  • Determined the limit of detection (LOD) and limit of quantification (LOQ) using reference materials.
  • Quantified fish DNA in a blended food matrix and analyzed commercial food samples.

Main Results:

  • The PCR method demonstrated high specificity for 20 Mediterranean fish species.
  • Achieved an LOD of 0.5 pg/µL and an LOQ of 5 pg/µL.
  • Successfully detected fish DNA at concentrations as low as 0.000025% (0.25 mg/kg) in a food blend.
  • Detected fish DNA in commercial samples, including those with undeclared or trace amounts of fish.

Conclusions:

  • The validated real-time PCR method is reliable, specific, and sensitive for fish DNA detection in foods.
  • This method can effectively support the verification of allergen labeling regulations.
  • The assay is a valuable tool for ensuring consumer safety regarding fish allergies.