Multifaceted effects of difenoconazole in tomato fruit ripening: Physiology, flavour and nutritional quality

Difenoconazole is widely used in crop growth, however, its effects on the quality of agricultural products are poorly studied. In this study, the application of difenoconazole on tomato plants could increase soluble sugar content, reduce organic acid and raise accumulation of nutrient-related metabolites during late fruit ripening. Consumer surveys in our study showed that the treatment of difenoconazole tomatoes group had higher sweetness and lower acidity, and those tomatoes were preferred by consumers. Alterations in fruit flavor-related attributes were at least in part corroborated by the abundance of transcripts related to sucrose (SlLin5, SlLin7, SlSuS2, SlSuS6, SlSPS1, SlSPS3) and organic acids (CS, ICDH, cMDH) anabolism. Furthermore, the difenoconazole also significantly promoted the expression of phytohormones synthesis genes, and consequently increased abscisic acid and ethylene levels. Our study not only provides theoretical support for the use of difenoconazole on tomatoes at the level of flavor quality and nutritional health, but also provides valuable information on the mechanism of triazole fungicides in the flavor quality of tomato fruits.