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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

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Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
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MicroRNAs01:22

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
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Performing Custom MicroRNA Microarray Experiments
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MicroRNA Expression Profiling Using Agilent One-Color Microarray.

Carmela Dell'Aversana1,2, Giulia Sgueglia3, Nunzio Del Gaudio3

  • 1Institute Experimental Endocrinology and Oncology 'Gaetano Salvatore' (IEOS)-National Research Council (CNR), Naples, Italy. carmela.dellaversana@cnr.it.

Methods in Molecular Biology (Clifton, N.J.)
|November 28, 2022
PubMed
Summary
This summary is machine-generated.

MicroRNA (miRNA) expression profiling identifies miRNA regulation in health and disease. This study details microarray methods using Agilent SurePrint technology for accurate miRNA analysis.

Keywords:
Expression profileMicroarrayPlatformSlidemiRNA

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Area of Science:

  • Genomics
  • Molecular Biology
  • Biotechnology

Background:

  • MicroRNA (miRNA) expression profiling is crucial for understanding biological regulation in various states.
  • miRNA analysis has significant applications in molecular diagnostics for diseases like cancer and in forensics.
  • Numerous high-throughput genomic techniques exist for miRNA profiling.

Purpose of the Study:

  • To describe the workflow and methods for miRNA expression profiling.
  • To focus on microarray-based profiling using Agilent SurePrint technology.

Main Methods:

  • Utilizing Agilent One-Color Microarray technology for miRNA expression profiling.
  • Detailed description of the experimental workflow and analytical methods.

Main Results:

  • Provides a comprehensive overview of the Agilent One-Color Microarray workflow for miRNA analysis.
  • Demonstrates the utility of SurePrint technology for accurate miRNA expression profiling.

Conclusions:

  • Agilent One-Color Microarray with SurePrint technology is an effective method for miRNA expression profiling.
  • This technique supports diverse applications in diagnostics and research.