3-D H-scan ultrasound imaging of relative scatterer size using a matrix array transducer and sparse random aperture...

H-scan ultrasound (US) is a high-resolution imaging technique for soft tissue characterization. By acquiring data in volume space, H-scan US can provide insight into subtle tissue changes or heterogenous patterns that might be missed using traditional cross-sectional US imaging approaches. In this study, we introduce a 3-dimensional (3-D) H-scan US imaging technology for voxel-level tissue characterization in simulation and experimentation. Using a matrix array transducer, H-scan US imaging was developed to evaluate the relative size of US scattering aggregates in volume space. Experimental data was acquired using a programmable US system (Vantage 256, Verasonics Inc, Kirkland, WA) equipped with a 1024-element (32 × 32) matrix array transducer (Vermon Inc, Tours, France). Imaging was performed using the full array in transmission. Radiofrequency (RF) data sequences were collected using a sparse random aperture compounding technique with 6 different data compounding approaches. Plane wave imaging at five angles was performed at a center frequency of 8 MHz. Scan conversion and attenuation correction were applied. To generate the 3-D H-scan US images, a convolution filter bank (N = 256) was then used to process the RF data sequences and measure the spectral content of the backscattered US signals before volume reconstruction. Preliminary experimental studies were conducted using homogeneous phantom materials embedded with spherical US scatterers of varying diameter, i.e., 27 to 45, 63 to 75, or 106-126 μm. Both simulated and experimental results revealed that 3-D H-scan US images have a low spatial variance when tested with homogeneous phantom materials. Furthermore, H-scan US is considerably more sensitive than traditional B-mode US imaging for differentiating US scatterers of varying size (p = 0.001 and p = 0.93, respectively). Overall, this study demonstrates the feasibility of 3-D H-scan US imaging using a matrix array transducer for tissue characterization in volume space.