Specifically functionalized MTT-Ag NP/SA film sensor for the ultrasensitive detection of Hg²⁺ in lettuce samples

In the present study, highly efficient 5-Methyl-1,3,4-thiadiazole-2-thiol-modified silver nanoparticles (MTT-Ag NPs) were successfully synthesized and could be used for convenient and sensitive detection of Hg²⁺. MTT acts as a protective agent by forming Ag-S bonds with Ag NPs, meantime, MTT can also be captured Hg²⁺ through NN bonds. Furthermore, to improve the sustainability and stability of MTT-Ag NPs, sodium alginate (SA) was used as a substrate material for the formation of SA-MTT-Ag NPs films. As expected, SA-MTT-Ag NPs could be stored for more than 180 days at room temperature. When used SA-MTT-Ag NPs thin films as colourimetric sensors for detection of Hg²⁺ in lettuce, the low detection limit could be down to 0.22 μM (44 ppb) with wide linear range (0-1 µM and 1-150 µM) and good recovery (96.25 % - 98.75 %). Therefore, the method enables highly selective and efficient monitoring of Hg²⁺ in food samples.