Related Experiment Video
Updated: Aug 19, 2025
Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications
Published on: June 23, 2016
A portable paper-based aptasensor for simultaneous visual detection of two mycotoxins in corn flour using dual-color
Xianfeng Lin1, Changxin Li1, Xinyu Tong1
1State Key Laboratory of Food Science and Technology, School of Food Science and Technology, International Joint Laboratory on Food Safety, Jiangnan University, Wuxi 214122, China.
Abstract:
A portable paper-based microfluidic aptasensor is established to simultaneously and visually detect zearalenone (ZEN) and ochratoxin A (OTA). The targets at the sample zone can migrate to two detection zones through dual-channels and result in green and blue fluorescence recovery. This is due to the specific recognition by a respective aptamer that destroys fluorescence resonance energy transfer (FRET) from dual-color upconversion nanoparticles (UCNPs) to Cu-TCPP nanosheets. By capturing fluorescent images and analyzing the corresponding RGB value via a smartphone, ZEN and OTA can be analyzed with limits of detection down to 0.44 ng/mL and 0.098 ng/mL in the linear ranges of 0.5-100 ng/mL and 0.1-50 ng/mL, respectively. Satisfactory recoveries are also obtained for ZEN (94.5-103.7 %) and OTA (92.2-106.8 %) in corn flour. With the advantages of simple operation, low sample consumption, and broad adaptability, this promising platform allows for the on-site detection of multiple hazards in food.
